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JoVE Journal
Immunology and Infection

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原位检测自身反应性CD4 + T细胞在大脑和心脏使用主要组织相容性复合II类Dextramers的
 
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原位检测自身反应性CD4 + T细胞在大脑和心脏使用主要组织相容性复合II类Dextramers的

Article DOI: 10.3791/51679-v 13:10 min August 1st, 2014
August 1st, 2014

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Summary

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Click here for the English version.

通过直接染色与主要组织相容性复合物Ⅱ类dextramers检测自身反应性CD4 + T细胞在大脑和心脏的协议已经本报告中进行了描述。为综合分析,一种可靠的方法来枚举抗原特异性CD4 + T细胞在原位的频率也被设计出来。

Tags

免疫学,第90期,dextramers; MHCⅡ类;
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