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Journal / Immunology and Infection / In Situ Påvisning af autoreaktive CD4 T-celler i…
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Immunology and Infection

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JoVE Journal Immunology and Infection
In Situ Påvisning af autoreaktive CD4 T-celler i hjernen og hjertet Brug hovedhistokompatibilitetskompleks klasse II Dextramers
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In Situ Påvisning af autoreaktive CD4 T-celler i hjernen og hjertet Brug hovedhistokompatibilitetskompleks klasse II Dextramers

Article DOI: 10.3791/51679-v • 13:10 min • August 1st, 2014
August 1st, 2014 •
1, 1, 1, 2, 3, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, 2Center for Biotechnology, University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln

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Protokollen til påvisning selvnedbrydende CD4 T-celler i hjernen og hjertet ved direkte farvning med større histokompatibilitetskompleks klasse II dextramers er blevet beskrevet i denne rapport. For omfattende analyse, en pålidelig metode til at opregne de frekvenser af antigen-specifikke CD4 + T-celler i situ også udtænkt.

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Immunologi dextramers; MHC klasse II;
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