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Journal / Immunology and Infection / A detecção in situ de células T CD4 autoreactive…
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Immunology and Infection

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JoVE Journal Immunology and Infection
A detecção in situ de células T CD4 autoreactive em Cérebro e Coração Usando principal de histocompatibilidade Dextramers classe II
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A detecção in situ de células T CD4 autoreactive em Cérebro e Coração Usando principal de histocompatibilidade Dextramers classe II

Article DOI: 10.3791/51679-v • 13:10 min • August 1st, 2014
August 1st, 2014 •
1, 1, 1, 2, 3, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, 2Center for Biotechnology, University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln

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O protocolo para detectar células auto-reactivas T CD4 no cérebro e no coração por coloração direta com complexo de histocompatibilidade principal dextramers classe II foi descrito neste relatório. Para análise detalhada, um método confiável para enumerar as freqüências de células T CD4 + antígeno-específicas in situ também é concebido.

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Imunologia Edição 90 dextramers; MHC classe II;
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