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Journal / Immunology and Infection / In-situ-Nachweis autoreaktiver CD4-T-Zellen in…
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Immunology and Infection

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JoVE Journal Immunology and Infection
In-situ-Nachweis autoreaktiver CD4-T-Zellen in Gehirn und Herz Mit Haupthistokompatibilitätskomplex Klasse II Dextramers
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In-situ-Nachweis autoreaktiver CD4-T-Zellen in Gehirn und Herz Mit Haupthistokompatibilitätskomplex Klasse II Dextramers

Article DOI: 10.3791/51679-v • 13:10 min • August 1st, 2014
August 1st, 2014 •
1, 1, 1, 2, 3, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, 2Center for Biotechnology, University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln

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Das Protokoll zur selbst-reaktiven CD4-T-Zellen erkennen in Gehirn und Herz durch direkte Färbung mit Haupthistokompatibilitätskomplex Klasse II dextramers hat in diesem Bericht beschrieben. Für Analysen, ist eine zuverlässige Methode, um die Frequenzen von Antigen-spezifischen CD4 + T-Zellen in situ aufzuzählen auch entwickelt.

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Immunologie dextramers; MHC-Klasse-II;
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