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快速分离纯化线粒体移植用组织分离和鉴别过滤
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JoVE Journal Biology
Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
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快速分离纯化线粒体移植用组织分离和鉴别过滤

DOI:
10.3791/51682-v

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10:35 min

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September 06, 2014

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, , , , ,
1Division of Cardiothoracic Surgery,Beth Israel Deaconess Medical Center and Harvard Medical School, 2Department of Anesthesiology, Perioperative and Pain Medicine,Boston Children’s Hospital and Department of Anesthesia, Harvard Medical School

Chapters

  • 00:05Title
  • 01:21Harvesting Mitochondria
  • 04:32The ATP Assay
  • 08:18Mitochondria Characterization from Liver and Skeletal Muscle Biopsy
  • 10:16Conclusion

Summary

Automatic Translation

Automatic Translation

一种用于线粒体的从哺乳动物组织活检快速分离方法进行说明。大鼠肝或骨骼肌肉制剂匀浆与商业组织离解和线粒体是通过尼龙网过滤分离差过滤。线粒体隔离时间是相对于60 <30分钟 - 用替代的方法100分钟。

Tags

Mitochondrial IsolationRapid IsolationDifferential FiltrationBiopsyNylon Mesh FiltersRespiration Competent Mitochondria

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