Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Hurtig isolering og oprensning af mitokondrier…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Hurtig isolering og oprensning af mitokondrier til transplantation Ved Tissue Dissociation og differentieret Filtrering
Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
Journal (Biology)
Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
Journal (Biology)
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
 
Click here for the English version

Hurtig isolering og oprensning af mitokondrier til transplantation Ved Tissue Dissociation og differentieret Filtrering

Article DOI: 10.3791/51682-v • 10:35 min • September 6th, 2014
September 6th, 2014 •
1, 2, 1, 2, 2, 1
1Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, 2Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital and Department of Anesthesia, Harvard Medical School

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

En metode til hurtig isolering af mitokondrier fra pattedyr vævsbiopsier beskrives. Rotte lever eller skeletmuskulaturen præparater blev homogeniseret med en kommerciel væv dissociator og mitokondrier blev isoleret ved differential filtrering gennem nylon mesh filtre. Mitokondrie isolation tid er <30 min sammenlignet med 60-100 minutter ved hjælp af alternative metoder.

Tags

Cellebiologi kirurgiske procedurer operative efterforskningsteknikker mitokondrier isolering filtrering homogenatet væv dissociation transplantation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter