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In Vivo 4-Dimensional Sporing av Hematopoietiske Stem og stamceller i Adult Mouse Calvarial Bone Marrow
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In Vivo 4-Dimensional Sporing av Hematopoietiske Stem og stamceller i Adult Mouse Calvarial Bone Marrow

Article DOI: 10.3791/51683-v • 12:54 min • September 4th, 2014
September 4th, 2014 •
1, 2, 2
1Department of Life Science and Facility for Imaging by Light Microscopy, Imperial College London, 2Department of Life Sciences, Imperial College London

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Naturen av samspillet mellom blodkreft stamceller og stamceller (HSPCs) og beinmargs nisjer er dårlig forstått. Tilpassede hardware modifikasjoner og en multi-trinn oppkjøpet protokollen tillater bruk av to-foton og konfokalmikroskopi til bilde ex vivo merket HSPCs omplassert innen benmargs områder, sporing samhandling og bevegelse.

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Cellular Biology stamcelle multiphoton mikroskopi celle sporing benmarg nisje calvarium intra-vital konfokalmikroskopi time-lapse imaging multi-modal mikros
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