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In Vivo Rastreamento 4-Dimensional de tronco hematopoéticas e células progenitoras em adultos mouse na calota craniana de Medula Óssea
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In Vivo Rastreamento 4-Dimensional de tronco hematopoéticas e células progenitoras em adultos mouse na calota craniana de Medula Óssea

Article DOI: 10.3791/51683-v • 12:54 min • September 4th, 2014
September 4th, 2014 •
1, 2, 2
1Department of Life Science and Facility for Imaging by Light Microscopy, Imperial College London, 2Department of Life Sciences, Imperial College London

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A natureza das interações entre células tronco e progenitoras hematopoéticas (hspCs) e nichos de medula óssea é pouco compreendido. Modificações de hardware personalizado e um protocolo de aquisição multi-passo permite o uso de dois fótons e microscopia confocal a imagem ex vivo hspCs rotulados hospedados dentro de áreas da medula óssea, as interações de rastreamento e movimento.

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Biologia Celular Edição 91 células-tronco hematopoéticas microscopia multiphoton rastreamento de celular nicho de medula óssea calvarium microscopia confocal intra-vital imagem time-lapse microscopia multi-modal
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