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JoVE Journal
Biology

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产生和人类INO80染色质重塑复合物及其亚纯化
 
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产生和人类INO80染色质重塑复合物及其亚纯化

Article DOI: 10.3791/51720
October 23rd, 2014

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Summary October 23rd, 2014

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这个协议描述了用于产生和纯化野生型和人INO80染色质重构复合物的突变版本中的一个过程。表位标记的INO80亚基的版本都稳定地表达在HEK293细胞中,并完全配合物和配合物缺乏亚基的特定集合是通过免疫亲和层析纯化。

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