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JoVE Journal
Biology

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产生和人类INO80染色质重塑复合物及其亚纯化
 
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产生和人类INO80染色质重塑复合物及其亚纯化

Article DOI: 10.3791/51720-v 08:44 min October 23rd, 2014
October 23rd, 2014

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Summary

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这个协议描述了用于产生和纯化野生型和人INO80染色质重构复合物的突变版本中的一个过程。表位标记的INO80亚基的版本都稳定地表达在HEK293细胞中,并完全配合物和配合物缺乏亚基的特定集合是通过免疫亲和层析纯化。

Tags

生物化学,第92期,染色质重塑,INO80,SNF2家庭ATP酶,结构与功能,酶的纯化
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