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Generation og oprensning af human INO80 Chromatin Remodeling komplekser og Subcomplexes
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Generation og oprensning af human INO80 Chromatin Remodeling komplekser og Subcomplexes

Article DOI: 10.3791/51720-v • 08:44 min • October 23rd, 2014
October 23rd, 2014 •
1,2, 1, 1,2, 1,2
1Stowers Institute for Medical Research, 2Department of Biochemistry & Molecular Biology, Kansas University Medical Center

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Denne protokol beskriver en fremgangsmåde til generering og oprensning af vildtype og mutant-versioner af humane INO80 kromatin remodellering kompleks. Epitop taggede versioner af INO80 underenheder stabilt udtrykt i HEK293-celler, og komplette komplekser og komplekser mangler specifikke sæt af underenheder oprenses ved immunaffinitetskromatografi.

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Biokemi kromatin remodellering INO80 SnF2 familie ATPase struktur-funktion enzymoprensning
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