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In Vitro Pancreas Organogenesis fra spredte Mouse Embryonale Progenitorer

Article DOI: 10.3791/51725-v • 08:35 min • July 19th, 2014
July 19th, 2014 •
*1, *1, *2, 1,2
1Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Swiss Institute for Experimental Cancer Research, 2DanStem, University of Copenhagen
* These authors contributed equally

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Den tredimensionale kultur beskrevet i denne protokol metode sammenfatter bugspytkirtlen udvikling fra spredte embryonale mus bugspytkirtel stamfædre, herunder deres betydelige ekspansion, differentiering og morfogenese i en forgrenet orgel. Denne metode er modtagelig for billedbehandling, funktionelle indblanding og manipulation af den niche.

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Developmental Biology bugspytkirtel stamceller Forgrening epitel udvikling Orgel Kultur 3D Kultur Diabetes differentiering Morfogenese Cell organisation beta-celle.
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