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Journal / Neuroscience / Stab Wound Injury of the Zebrafish Adult…
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Neuroscience

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JoVE Journal Neuroscience
Stab Wound Injury of the Zebrafish Adult Telencephalon
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Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration

Article DOI: 10.3791/51753-v • 09:16 min • August 4th, 2014
August 4th, 2014 •
1, 1, 1, 1
1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology

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Summary
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To shed light on the cellular and molecular mechanisms of zebrafish adult neurogenesis and regeneration, we developed a protocol for invasive surgery causing mechanical injuries in the zebrafish adult telencephalon and subsequent monitoring of changes in the stabbed hemisphere by immunohistochemistry or in situ hybridization.

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Stab Wound Injury Zebrafish Adult Telencephalon Investigate Vertebrate Brain Neurogenesis Regeneration Cellular Response Molecular Mechanisms CNS Regeneration Repair Zebrafish Model Insulin Syringe Needle Post Injury Days Fish Sacrifice Brain Dissection Immunohistochemistry In Situ Hybridization (ISH) Cell Proliferation Gliogenesis Neurogenesis Internal Control RNA Deep Sequencing New Genes
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