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Zink-finger Nuclease Forbedret genmålretning i humane embryonale stamceller
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Zink-finger Nuclease Forbedret genmålretning i humane embryonale stamceller

Article DOI: 10.3791/51764-v • 12:13 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1, 1, 1
1Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University

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Reporter cellelinjer tilbyder et middel til at visualisere, spore og isolere celler af interesse fra heterogene populationer. Men gen-targeting anvendelse af konventionel homolog rekombination i humane embryonale stamceller er yderst ineffektivt. Heri beskriver vi målrettet CNS midthjernen specifik transkriptionsfaktor PITX3 locus med EGFP hjælp zink-finger nuklease forbedret homolog rekombination.

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Molekylærbiologi Elektroporation humane embryonale stamceller genom redigering reporter cellelinie midthjernen dopaminerge neuroner
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