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Sink-finger Nuclease Forbedret Gene Targeting i humane embryonale stamceller
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Sink-finger Nuclease Forbedret Gene Targeting i humane embryonale stamceller

Article DOI: 10.3791/51764-v • 12:13 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1, 1, 1
1Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University

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Rapportørcellelinjer gir et middel for å visualisere, spore og isolere celler av interesse fra heterogene populasjoner. Det er imidlertid gene-rettet ved hjelp av konvensjonelle homolog rekombinasjon i humane embryoniske stamceller ekstremt ineffektiv. Heri beskriver vi målretting CNS midthjernen spesifikk transkripsjonsfaktor PITX3 locus med EGFP bruker sink-finger nuclease forbedret homolog rekombinasjon.

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Molecular Biology Electroporation humane embryonale stamceller genom redigering reporter cellelinje midthjernen dopaminerge nevroner
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