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Journal / Biology / Un protocollo video di infezione retrovirale in…
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Un protocollo video di infezione retrovirale in Primaria intestinale organoide Cultura
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Un protocollo video di infezione retrovirale in Primaria intestinale organoide Cultura

Article DOI: 10.3791/51765-v • 09:18 min • August 11th, 2014
August 11th, 2014 •
1,2, 1,2, 2, 1,2
1Department of Genetics, University of Cambridge, 2Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge

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Questo protocollo spiega cultura organoide Lgr5-positivo primaria e la successiva esecuzione di trasduzione retrovirale. Ciò consente sovraespressione Cre-inducibile o knockdown del transgene consegnato e permette studi funzionali da realizzare nel romanzo in vitro organotipica sistema modello.

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Genetica Retrovirus Lentivirus cultura organoide Lgr5 Intestino 3R
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