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En video-protokollen af ​​retroviral infektion i Primary Intestinal organoide Kultur
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En video-protokollen af ​​retroviral infektion i Primary Intestinal organoide Kultur

Article DOI: 10.3791/51765-v • 09:18 min • August 11th, 2014
August 11th, 2014 •
1,2, 1,2, 2, 1,2
1Department of Genetics, University of Cambridge, 2Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge

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Denne protokol forklarer primær Lgr5-positve organoide kultur og den efterfølgende udførelse af retroviral transduktion. Dette muliggør Cre-inducerbar overekspression eller knockdown af den leverede transgen og tillader funktionelle undersøgelser, der skal gennemføres i romanen in vitro organotypisk modelsystem.

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Genetik retrovirus lentivirus organoide kultur Lgr5 tarm 3R
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