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Un protocole de vidéo infections rétrovirales dans intestinale primaire Organoid Culture
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Un protocole de vidéo infections rétrovirales dans intestinale primaire Organoid Culture

Article DOI: 10.3791/51765-v • 09:18 min • August 11th, 2014
August 11th, 2014 •
1,2, 1,2, 2, 1,2
1Department of Genetics, University of Cambridge, 2Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge

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Ce protocole explique culture organoïde primaire LGR5-positve et la performance ultérieure de transduction rétrovirale. Cela permet la surexpression Cre inductible ou effet de choc du transgène livré et permet des études fonctionnelles à réaliser dans le roman en organotypique in vitro système modèle.

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Génétique Numéro 90 rétrovirus lentivirus culture Organoid LGR5 Intestin 3R
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