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Un Protocolo de vídeo de la infección retroviral en Primaria Intestinal organoide Cultura
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Un Protocolo de vídeo de la infección retroviral en Primaria Intestinal organoide Cultura

Article DOI: 10.3791/51765-v • 09:18 min • August 11th, 2014
August 11th, 2014 •
1,2, 1,2, 2, 1,2
1Department of Genetics, University of Cambridge, 2Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge

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Este protocolo explica cultura organoid-Lgr5 positve primaria y la posterior ejecución de la transducción retroviral. Esto permite la sobreexpresión Cre-inducible o caída del transgén entregado y permite estudios funcionales que se llevarán a cabo en la novela en organotípico vitro sistema modelo.

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Genética Retrovirus Lentivirus cultura organoide Lgr5 Intestino 3Rs
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