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Immunology and Infection

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JoVE Journal Immunology and Infection
Real-time Imaging av endotelceller-celle Junctions Under Neutrophil Sjele under fysiologiske Flow
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Real-time Imaging av endotelceller-celle Junctions Under Neutrophil Sjele under fysiologiske Flow

Article DOI: 10.3791/51766-v • 11:26 min • August 14th, 2014
August 14th, 2014 •
*1, *1, 1, 1
1Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam
* These authors contributed equally

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Leukocytter krysse endothelial monolayer bruker paracellular eller transcellular rute. Vi har utviklet en enkel analyse for å følge den fordeling av endogent junctional VE-cadherin og PECAM-1 under leukocytt transendothelial migrasjon under fysiologiske strømmen til å diskriminere mellom de to Sjele ruter.

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Immunologi leukocytter menneskelige umbilical vein endotelceller (HUVECs) sjelevandring VE-cadherin PECAM-1 endotelet transcellular paracellular
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