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JoVE Journal Bioengineering
En Mikrofluid baseret Elektrokemisk Bioflis efter etiket-fri DNA-hybridisering Analyse
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En Mikrofluid baseret Elektrokemisk Bioflis efter etiket-fri DNA-hybridisering Analyse

Article DOI: 10.3791/51797-v • 14:53 min • September 10th, 2014
September 10th, 2014 •
1, 1, 2, 2, 1
1MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, 2Institute for Bioscience and Biotechnology Research, Fischell Department of Bioengineering, University of Maryland

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Vi præsenterer en mikrofluidindretning-baserede elektrokemisk biochip for DNA hybridisering detektion. Efter ssDNA sonde funktionalisering er specificitet, sensitivitet, og detektionsgrænse den studerede med komplementære og ikke-komplementære ssDNA mål. Resultaterne viser indflydelsen af ​​DNA-hybridisering begivenheder på den elektrokemiske system, med en detektionsgrænse på 3,8 nM.

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Bioengineering elektrokemisk impedans spektroskopi DNA-hybridisering biosensorer biochippen mikrofluidik etiket-fri detektion begrænset diffusion mikrofabrikation
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