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JoVE Journal Bioengineering
En microfluidic basert Elektrokjemisk biochip for Label-free DNA Hybridisering Analyse
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En microfluidic basert Elektrokjemisk biochip for Label-free DNA Hybridisering Analyse

Article DOI: 10.3791/51797-v • 14:53 min • September 10th, 2014
September 10th, 2014 •
1, 1, 2, 2, 1
1MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, 2Institute for Bioscience and Biotechnology Research, Fischell Department of Bioengineering, University of Maryland

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Vi presenterer en mikrofluidbasert elektro biochip for DNA hybridisering deteksjon. Etter ssDNA probe funksjon, er spesifisiteten, følsomhet, og deteksjonsgrense studert med komplementære og ikke-komplementære ssDNA mål. Resultatene illustrerer påvirkning av DNA-hybridise-hendelsene på elektrokjemisk system, med en deteksjonsgrense på 3,8 nM.

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Bioteknologi elektro impedansspektroskopi DNA hybridisering biosensor biochip MicroFluidics etikett-free deteksjon begrenset diffusjon microfabrication
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