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JoVE Journal Bioengineering
En mikroflödesbaserad Elektro Biochip för Label-fritt DNA hybridisering Analys
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En mikroflödesbaserad Elektro Biochip för Label-fritt DNA hybridisering Analys

Article DOI: 10.3791/51797-v • 14:53 min • September 10th, 2014
September 10th, 2014 •
1, 1, 2, 2, 1
1MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, 2Institute for Bioscience and Biotechnology Research, Fischell Department of Bioengineering, University of Maryland

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Vi presenterar en mikroflödesbaserad elektro biochip för DNA-hybridisering detektering. Efter ssDNA sond funktion är specificiteten, känslighet och detektionsgräns studeras med kompletterande och icke-komplementära ssDNA mål. Resultaten illustrerar inverkan av DNA-hybridiserings-händelser på den elektrokemiska systemet, med en detektionsgräns av 3,8 nM.

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Bioteknik elektrokemisk impedansspektroskopi DNA-hybridisering biosensor biochip mikrofluidik etikett-fri upptäckt begränsad diffusion mikrofabrikation
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