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13:26 min
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September 13, 2014
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Summary
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Differential scanning fluorimetry is a widely used method for screening libraries of small molecules for interactions with proteins. Here, we present a straightforward method to extend these analyses to provide an estimate of the dissociation constant between a small molecule and its protein partner.
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Cite this Article
Vivoli, M., Novak, H. R., Littlechild, J. A., Harmer, N. J. Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry. J. Vis. Exp. (91), e51809, doi:10.3791/51809 (2014).
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