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Journal / Biology / Cytosoliske Calcium Målinger i Nyrer epitelceller …
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Cytosoliske Calcium Målinger i Nyrer epitelceller ved flowcytometri
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Cytosoliske Calcium Målinger i Nyrer epitelceller ved flowcytometri

Article DOI: 10.3791/51857-v • 10:24 min • October 28th, 2014
October 28th, 2014 •
1, 2
1Institute for Physiology, Pathophysiology, & Toxicology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke, 2Institute for Immunology & Experimental Oncology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke

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Calcium er involveret i mange fysiologiske og patofysiologiske signalveje. Live-cell imaging kræver specialiseret udstyr og kan være tidskrævende. En hurtig, enkel metode under anvendelse af et flowcytometer til at bestemme de relative ændringer i cytosolisk calcium i adhærerende epitelceller bragt i suspension blev optimeret.

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Cellular Biology Nyre FACS andet messenger proksimal tubulus calcium indikatorer probenecid endoplasmatisk reticulum ionomycin
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