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Cytosole Calcium Metingen in Renal epitheelcellen door flowcytometrie
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Cytosole Calcium Metingen in Renal epitheelcellen door flowcytometrie

Article DOI: 10.3791/51857-v • 10:24 min • October 28th, 2014
October 28th, 2014 •
1, 2
1Institute for Physiology, Pathophysiology, & Toxicology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke, 2Institute for Immunology & Experimental Oncology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke

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Calcium is betrokken bij tal van fysiologische en pathofysiologische signaalwegen. Live cell imaging vereist gespecialiseerde apparatuur en kan tijdrovend zijn. Een snelle, eenvoudige werkwijze met een flow cytometer om relatieve veranderingen in cytosolische calcium in hechtende epitheelcellen gebracht suspensie bepalen geoptimaliseerd.

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Cellular Biology Nier FACS tweede boodschapper proximale tubulus calcium indicatoren probenecide endoplasmatisch reticulum ionomycine
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