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JoVE Journal Bioengineering
En ny metode til lokalisering Reporter fluorescerende kugler nærheden cellekulturen overflade for trækkraft Microscopy
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En ny metode til lokalisering Reporter fluorescerende kugler nærheden cellekulturen overflade for trækkraft Microscopy

Article DOI: 10.3791/51873-v • 13:30 min • September 16th, 2014
September 16th, 2014 •
1, 1, 1
1Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign

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Traditionelle teknikker til fremstilling af polyacrylamid (PA), geler, der indeholder fluorescerende prober indebærer sandwiching en gel mellem en Tilhænger overflade og en glasplade. Her viser vi, at overtrække denne objektglas med poly-D-lysin (PDL) og fluorescerende prober lokaliserer prober inden for 1,6 um fra geloverfladen.

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Bioengineering celle mekanik polyacrylamid (PA) gel trækkraft mikroskopi fluorescerende perler poly-D-lysin (PDL) cellekultur overflade
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