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Bioengineering

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JoVE Journal Bioengineering
En ny metode for lokalisering Reporter fluoriserende perler Nær Cell Culture Surface for trekkraft Mikros
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En ny metode for lokalisering Reporter fluoriserende perler Nær Cell Culture Surface for trekkraft Mikros

Article DOI: 10.3791/51873-v • 13:30 min • September 16th, 2014
September 16th, 2014 •
1, 1, 1
1Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign

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Tradisjonelle teknikker for å fabrikkere polyakrylamid (PA) geler inneholdende fluorescerende prober innebære sandwiching en gel mellom en vedheftende overflate, og en glass-slide. Her viser vi at belegg dette lysbildet med poly-D-lysin (PDL) og fluorescerende prober lokaliserer sondene til innenfor 1,6 mikrometer fra gelen overflaten.

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Bioteknologi celle mekanikk polyakrylamid (PA) gel trekkraft mikroskopi fluoriserende perler poly-D-lysin (PDL) cellekultur overflaten
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