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JoVE Journal
Neuroscience

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Rápido Genotipado de animales seguidas del establecimiento de cultivos primarios de neuronas cerebrales

Article DOI: 10.3791/51879
January 29th, 2015 •
*1,2, *1, 3, 1
1Department of Molecular Physiology & Biophysics, University of Iowa Carver College of Medicine, 2Department of Psychiatry, University of Iowa Carver College of Medicine, 3EZ BioResearch LLC
* These authors contributed equally

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January 29th, 2015

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Se describe procedimientos para el etiquetado y la genotipificación ratones recién nacidos y la generación de cultivos primarios de neuronas de ellos. La genotipificación es rápida, eficiente y fiable, y permite la extracción automatizada de ácido nucleico. Esto es especialmente útil para los ratones neonatally letales y sus culturas que requieren previa cumplimentación del genotipado.

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