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JoVE Journal Neuroscience
Fremstilling af Synaptic plasmamembranen og postsynaptiske Density Proteiner hjælp af en Diskontinuerlig sucrosegradient
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Fremstilling af Synaptic plasmamembranen og postsynaptiske Density Proteiner hjælp af en Diskontinuerlig sucrosegradient

Article DOI: 10.3791/51896-v • 08:06 min • September 3rd, 2014
September 3rd, 2014 •
*1, *1, 1, 1
1Department of Pharmacology and Toxicology, University of Toronto
* These authors contributed equally

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Denne artikel beskriver berigelsen af ​​proteiner forbundet med den synaptiske plasmamembran ved ultracentrifugering på en diskontinuerlig saccharosegradient. Den efterfølgende udarbejdelse af postsynaptiske tæthed proteiner er også beskrevet. Protein præparater er egnede til western blotting eller 2D DIGE analyse.

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Neurobiologisk hjerne synapse western blot ultracentrifugering SPM PSD
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