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JoVE Journal
Biology

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非酶中,预浸润性乳腺癌病变的无血清组织培养的微球体的自然发生
 
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非酶中,预浸润性乳腺癌病变的无血清组织培养的微球体的自然发生

Article DOI: 10.3791/51926-v 09:49 min November 8th, 2014
November 8th, 2014

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Summary

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使用完整的组织组织体原代细胞培养提供了一个模型系统,模仿的多细胞在体内微环境。我们开发了一种无血清乳腺原发上皮细胞组织培养模式,延续了混合细胞培养系,并表现出分化的形态,没有酶的组织破坏。乳房组织体保持活力> 6个月。

Tags

癌症生物学,第93,乳腺,导管原位癌,表皮生长因子,微球体,类器官,浸润前,原代细胞培养,无血清,球体
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