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Niet-enzymatische, Serum-vrije Tissue Culture van Pre-invasieve borstlaesies voor Spontane Generatie van Mammospheres
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Niet-enzymatische, Serum-vrije Tissue Culture van Pre-invasieve borstlaesies voor Spontane Generatie van Mammospheres

Article DOI: 10.3791/51926-v • 09:49 min • November 8th, 2014
November 8th, 2014 •
1, 2, 1
1Center for Applied Proteomics and Molecular Medicine, George Mason University, 2Virginia Surgery Associates

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Primaire celcultuur met behulp van intact weefsel organoids biedt een modelsysteem dat de meercellige in vivo micro-omgeving nabootst. We ontwikkelden een serum-vrij primaire borst epitheel weefselkweek model dat gemengde celkweek geslachten en exposities gedifferentieerde morfologie bestendigt, zonder enzymatische weefsel verstoring. Borst organoids blijven nog gedurende> 6 maanden.

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Cancer Biology Borst ductaal carcinoma in situ epidermale groeifactor mammosphere organoïde pre-invasieve primaire celkweek serum-vrij spheroïde
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