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Ur 2DE-Gel Spot till Protein Funktion
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Ur 2DE-Gel Spot till Protein Funktion: Lesson Learned From HS1 i kronisk lymfatisk leukemi

Article DOI: 10.3791/51942-v • 10:18 min • October 19th, 2014
October 19th, 2014 •
1,2, 1, 3, 1, 1, 1,4, 1,4, 1
1Division of Molecular Oncology, IRCCS, San Raffaele Scientific Institute, 2Department of Haemato-Oncology, King's College London, 3IFOM, FIRC Institute of Molecular Oncology, 4Università Vita-Salute San Raffaele

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Här beskriver vi ett protokoll som kopplar två proteomik tekniker, nämligen 2-dimensionell elektrofores (2DE) och masspektrometri (MS), för att identifiera differentiellt uttryckta / posttranslation modifierade proteiner på två eller flera grupper av delprov. Detta tillvägagångssätt, tillsammans med funktionella experiment medger identifiering och karakterisering av prognostiska markörer / terapeutiska mål.

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Medicin lymfocyter kronisk lymfatisk leukemi 2D elektrofores masspektrometri Cytoskeleton migration
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