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Journal / Immunology and Infection / Utbredelsen av
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Immunology and Infection

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Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
Journal (Immunology and Infection)
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
Journal (Immunology and Infection)
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
 
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Utbredelsen av

Article DOI: 10.3791/51953-v • 12:52 min • September 25th, 2014
September 25th, 2014 •
1, 1, 2, 1
1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS

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Her presenterer vi en protokoll for å forplante Homalodisca vitripennis celler og HoCV-1 in vitro. Medium ble fjernet fra HoCV-1 positive kulturer og RNA ekstrahert hver 24. time i 168 timer. Cell overlevelsesevne ble kvantifisert ved trypanblått farging. Hele viruspartikler ble ekstrahert etter infeksjon. Ekstrahert RNA ble kvantifisert ved QRT-PCR.

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Infeksjon , Cellekultur Pierce sykdom av grapevine,
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