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Journal / Immunology and Infection / Formering af
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Immunology and Infection

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Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
Journal (Immunology and Infection)
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
Journal (Immunology and Infection)
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
 
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Formering af

Article DOI: 10.3791/51953-v • 12:52 min • September 25th, 2014
September 25th, 2014 •
1, 1, 2, 1
1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS

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Her præsenterer vi en protokol til at udbrede Homalodisca vitripennis celler og HoCV-1 in vitro. Medium blev fjernet fra HoCV-1-positive kulturer og RNA ekstraheres hver 24 timer til 168 timer. Cell overlevelsesevne blev kvantificeret ved trypanblåt-farvning. Hele viruspartikler blev ekstraheret efter infektion. Ekstraheret RNA blev kvantificeret ved QRT-PCR.

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Infektion , Cellekultur Pierce sygdom af Grapevine,
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