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Journal / Immunology and Infection / Yayılması
JoVE Journal
Immunology and Infection

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In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
Journal (Immunology and Infection)
In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
Journal (Immunology and Infection)
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
 
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Yayılması

Article DOI: 10.3791/51953-v • 12:52 min • September 25th, 2014
September 25th, 2014 •
1, 1, 2, 1
1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS

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Burada in vitro Homalodisca vitripennis hücreleri ve HoCV-1 yaymak için bir protokol mevcut. Orta HoCV-1 pozitif kültürlerden çıkarıldı ve RNA 168 saat süre ile, her 24 saat ekstre edilmiştir. Hücre yaşayabilirliği tripan mavi ile nicelendirildi. Tüm virüs parçacıkları sonrası enfeksiyon ekstre edildi. Çıkarılan RNA QRT-PCR ile belirlenmiştir.

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Enfeksiyon Sayı 91, Hücre kültürü asma Pierce hastalığı,
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