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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Trasfezione altamente efficiente di umani THP-1 da macrofagi Nucleofection
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Trasfezione altamente efficiente di umani THP-1 da macrofagi Nucleofection

Article DOI: 10.3791/51960
September 2nd, 2014 •
1, 1, 1
1Institute of Nutrition, Friedrich Schiller University Jena

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September 2nd, 2014

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Questo protocollo rappresenta un metodo efficiente e affidabile per transfettare umani THP-1 macrofagi con siRNA o il DNA plasmidico mediante elettroporazione con alta efficienza di trasfezione, pur mantenendo alta la vitalità delle cellule e la piena capacità dei macrofagi per la differenziazione e la polarizzazione.

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