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Journal / Immunology and Infection / Svært effektiv Transfeksjon menneskerettighets…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Svært effektiv Transfeksjon menneskerettighets THP-1 Makrofager av nucleofection
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Svært effektiv Transfeksjon menneskerettighets THP-1 Makrofager av nucleofection

Article DOI: 10.3791/51960-v • 07:54 min • September 2nd, 2014
September 2nd, 2014 •
1, 1, 1
1Institute of Nutrition, Friedrich Schiller University Jena

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Denne protokollen gir en effektiv og pålitelig metode for å transfektere humane THP-1 makrofager med siRNA eller plasmid DNA med elektroporering med høy transfeksjon effektivitet og samtidig opprettholde høy celle vitalitet og full makrofag kapasiteten for differensiering og polarisering.

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Infeksjon THP-1 makrofager transfeksjon elektroporering siRNA plasmid DNA protokoll polarisering nucleofection
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