An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, <em>Aedes albopictus</em>

Monica F. Poelchau; Xin Huang; Allison Goff; Julie Reynolds; Peter Armbruster

doi:10.3791/51961

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Een experimentele en Bioinformatica Protocol voor RNA-seq Analyses van Fotoperiodische diapause in de Aziatische tijgermug,<em> Aedes albopictus</em

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, <em>Aedes albopictus</em>

JoVE Journal
Biology

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JoVE Journal Biology

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Een experimentele en Bioinformatica Protocol voor RNA-seq Analyses van Fotoperiodische diapause in de Aziatische tijgermug,<em> Aedes albopictus</em

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13,287 Views

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12:10 min

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November 30, 2014

DOI:

10.3791/51961-v

DOI:

10.3791/51961-v

•

12:10 min

November 30, 2014

•

13266 Views

Monica F. Poelchau¹, Xin Huang¹, Allison Goff¹, Julie Reynolds², Peter Armbruster¹

¹Department of Biology,Georgetown University ²Insect Physiology Lab, EEOB,The Ohio State University

Summary

RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.