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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Article DOI: 10.3791/51993-v • 08:53 min • August 15th, 2014
August 15th, 2014 •
1, 1, 1
1Institute of Applied Research, Aalen University

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Summary
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A module for single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures. The sample is located within a rectangular capillary, and via a microfluidic system fluorescent dyes, pharmaceutical agents or drugs can be applied in small quantities.

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Single Plane Illumination Microscopy SPIM Light Sheet Microscopy Wide-field Microscope 3-dimensional Cell Cultures Multi-cellular Tumor Spheroids Capillary Approach Micro-capillary Fluorescence Detection Microfluidic System Fluorescent Dyes Pharmaceutical Agents Drugs Cytostatic Drug Doxorubicin Degradation Product Redox Measurements Genetically Encoded Glutathione Sensor Oxidizing Agent Cell Necrosis Mitochondrial Respiratory Chain
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