JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
在小鸡视网膜有效的基因转移的原代细胞培养研究:<em>前OVO</em>电穿孔法
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An
Ex-ovo
Electroporation Approach
Please note that all translations are automatically generated.
Click here for the English version.
在小鸡视网膜有效的基因转移的原代细胞培养研究:<em>前OVO</em>电穿孔法
DOI:
10.3791/52002-v
•
08:59 min
•
November 02, 2015
•
M. Natalia Vergara
,
Christian Gutierrez
,
M. Valeria Canto-Soler
1
Wilmer Eye Institute
,
The Johns Hopkins University School of Medicine
Chapters
00:05
Title
02:00
Preparation for Ex-ovo Electroporation
04:00
Collection of Retinal Cups from Chick Embryos
05:40
Electroporation
07:24
Results: Efficiency of Transgene Expression in Cultured Cells after Electroporation
08:10
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
鸡胚视网膜细胞培养物是宝贵的工具,用于感光生物学研究。我们已经开发了基于
前OVO
之前培养的视网膜质粒电高效的基因转移技术。这种技术显着地增加的转染效率比现有的协议,使遗传操作此系统是可行的。
Tags
Chick Retina
Primary Cell Culture
Ex-ovo Electroporation
Photoreceptor
Gene Transfer
Genetic Manipulation
Transfection
RNAi
Overexpression
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
优化<em>前OVO</em>鸡胚培养,以发展的高级阶段
小鼠胚胎植耳蜗的文化和基因转移通过电穿孔
评定神经连接的形成在共培养体系的光遗传学方法
正向遗传学的方法来揭开抗逆基因小鼠 - 在ES细胞高通量筛选
鸡小脑切片的
体外培养
和空间上有针对性的颗粒细胞前体电穿孔
基因转移到鸡听觉器官通过<em>在卵</em>微电
一本小说<em>防爆大毛</em>带技术来改变心内血流动力学在鸡胚胎系统
一种酶和无血清神经干细胞培养模型EMT研究适合药物发现
水下滤纸三明治长期<em>防爆大毛</em>早期鸡胚时间推移成像
分离的原发性上皮细胞从附睾的全细胞贴片钳记录
Read Article