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Diferenciação de alta eficiência de pluripotentes humanas células-tronco para Cardiomyocytes e Caracterização por citometria de fluxo
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Diferenciação de alta eficiência de pluripotentes humanas células-tronco para Cardiomyocytes e Caracterização por citometria de fluxo

Article DOI: 10.3791/52010-v • 13:13 min • September 23rd, 2014
September 23rd, 2014 •
*1, *2, 1, 1, 3, 2, 4,5, 1,6
1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin
* These authors contributed equally

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O artigo descreve a metodologia detalhada para diferenciar de forma eficiente as células estaminais pluripotentes em cardiomiócitos de modular selectivamente a via Wnt, seguido por análise de citometria de fluxo de marcadores de referência para avaliar a homogeneidade e a identidade da população.

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Biologia Celular Edição 91 induzida pelo homem de células-tronco pluripotentes citometria de fluxo a diferenciação dirigida cardiomiócitos IRX4 TNNI3 TNNT2 MCL2v MLC2a
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