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High Efficiency differentiatie van humane pluripotente stamcellen naar cardiomyocyten en karakterisering door flowcytometrie
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High Efficiency differentiatie van humane pluripotente stamcellen naar cardiomyocyten en karakterisering door flowcytometrie

Article DOI: 10.3791/52010-v • 13:13 min • September 23rd, 2014
September 23rd, 2014 •
*1, *2, 1, 1, 3, 2, 4,5, 1,6
1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin
* These authors contributed equally

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Het artikel beschrijft de gedetailleerde methodologie efficiënt onderscheiden humane pluripotente stamcellen naar cardiomyocyten door het selectief moduleren van de Wnt route, gevolgd door flow cytometrie analyse van referentiemarkeringen aan homogeniteit en identiteit van de bevolking beoordelen.

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Cellular Biology humane geïnduceerde pluripotente stamcellen flowcytometrie geregisseerd differentiatie cardiomyocyte IRX4 TNNI3 TNNT2 MCL2v MLC2a
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