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JoVE Journal
Developmental Biology

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Ekspression af fluorescerende proteiner i
 
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Ekspression af fluorescerende proteiner i

Article DOI: 10.3791/52042-v 09:31 min January 12th, 2015
January 12th, 2015

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Summary

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Click here for the English version.

Vi rapporterer her den robuste og effektiv ekspression af fluorescerende proteiner efter mRNA injektion i ubefrugtede oocytter fra Branchiostoma lanceolatum. Udviklingen af mikroinjektion teknik i denne basale chordate vil bane vejen for vidtgående tekniske innovationer i denne nye model, herunder in vivo imaging og gen-specifikke manipulationer.

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Developmental Biology amphioxus cephalochordate genekspression vektorer, mikroinjektion protokol modelorganisme
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