JoVE Journal
Developmental Biology
Developmental Biology
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蛍光タンパク質の発現において
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Article
DOI:
10.3791/52042-v
•
09:31 min
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January 12th, 2015
蛍光タンパク質の発現において
January 12th, 2015
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Estelle Hirsinger1, João Emanuel Carvalho2, Christine Chevalier1,3, Georges Lutfalla4, Jean-François Nicolas1, Nadine Peyriéras5, Michael Schubert2
1Département de Biologie du Développement et Cellules Souches, Institut Pasteur, 2Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universités, 3Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Cancérologie de Marseille, 4Unité de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Université Montpellier II, 5Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard
Chapters
Summary
Please note that all translations are automatically generated.
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私たちは、Branchiostomaのlanceolatumの未受精卵母細胞へのmRNA注入後、ここで蛍光タンパク質の堅牢かつ効率的な発現を報告している。この基底脊索動物におけるマイクロインジェクション技術の開発は、in vivoイメージングと遺伝子特異的な操作に含めて、この新たなモデル系で遠大な技術革新のための道を開く。