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Journal / Developmental Biology / Uttrykk for Fluorescent Proteiner i
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Developmental Biology

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JoVE Journal Developmental Biology
Uttrykk for Fluorescent Proteiner i
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Uttrykk for Fluorescent Proteiner i

Article DOI: 10.3791/52042-v • 09:31 min • January 12th, 2015
January 12th, 2015 •
1, 2, 1,3, 4, 1, 5, 2
1Département de Biologie du Développement et Cellules Souches, Institut Pasteur, 2Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universités, 3Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Cancérologie de Marseille, 4Unité de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Université Montpellier II, 5Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard

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Vi rapporterer her den robust og effektiv uttrykk for fluorescerende proteiner etter mRNA injeksjon i ubefruktede eggceller av Branchiostoma lanceolatum. Utviklingen av mikroinjeksjon teknikk i denne basal chordate vil bane vei for vidtrekkende tekniske nyvinninger i denne nye modellen systemet, inkludert in vivo avbildning og genspesifikke manipulasjoner.

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Developmental Biology amphioxus Lansettfisker genuttrykk vektorer, mikroinjeksjon protokollen modellorganisme
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