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1Department of Nanomedicine,Houston Methodist Research Institute, 2MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering,Sun Yat-sen University, 3Pediatrics Department of Union Hospital,Huazhong University of Science and Technology, 4CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety,National Center for Nanoscience & Technology of China, 5Department of Medicine,Weill Cornell Medical College, 6Department of Cell and Developmental Biology,Weill Cornell Medical College
Chapters
- 00:05Title
- 00:58PCPS Particle Preparation
- 03:46PCPS Particle Characterization
- 04:33Loading of siRNA into PCPS Particles and Optimization of siRNA/PCPS Particle Ratio
- 05:31Release of siRNA from PCPS Particles
- 06:16Results: Characterization of Porous Silicon Microparticles for siRNA Delivery
- 08:02Conclusion
Delivery remains the main challenge for the therapeutic implementation of small interfering RNA (siRNA). This protocol involves the use of a multifunctional and biocompatible siRNA delivery platform, consisting of arginine and polyethylenimine grafted porous silicon microparticles.
