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Journal / Developmental Biology / Storskalig Zebrafish Embryonala Hjärta Dissection …
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Developmental Biology

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JoVE Journal Developmental Biology
Storskalig Zebrafish Embryonala Hjärta Dissection för Transkriptionell Analys
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Storskalig Zebrafish Embryonala Hjärta Dissection för Transkriptionell Analys

Article DOI: 10.3791/52087-v • 10:00 min • January 12th, 2015
January 12th, 2015 •
*1,2,3, *1,2, 1,2,3
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology, University of Potsdam, 3Institute of Molecular Biology, Medizinische Hochschule Hannover
* These authors contributed equally

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För att analysera hjärt genuttrycksprofilerna under zebrafisk hjärta utveckling har totalt RNA extraheras från isolerade hjärtan. Här presenterar vi ett protokoll för insamling funktionella / slående hjärtan genom snabb manuell dissektion från zebrafisk embryon för att få hjärt-specifikt mRNA.

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Utvecklingsbiologi zebrafisk embryo hjärta dissektion RNA RT-qPCR
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