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Journal / Developmental Biology / 大规模的斑马鱼胚胎的心脏解剖的转录分析…
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大规模的斑马鱼胚胎的心脏解剖的转录分析
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大规模的斑马鱼胚胎的心脏解剖的转录分析

Article DOI: 10.3791/52087-v • 10:00 min • January 12th, 2015
January 12th, 2015 •
*1,2,3, *1,2, 1,2,3
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology, University of Potsdam, 3Institute of Molecular Biology, Medizinische Hochschule Hannover
* These authors contributed equally

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到斑马鱼心脏发育过程中分析的心脏基因表达分布，总RNA已被提取从离体心脏。在这里，我们提出了一个协议，用于收集功能/跳动的心迅速人工清扫，从斑马鱼胚胎获得心脏特异性mRNA。

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发育生物学，第95，斑马鱼胚胎，心脏，解剖，RNA，RT-qPCR的
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