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Journal / Developmental Biology / Großzebrafisch Embryonale Herz Dissection für…
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JoVE Journal Developmental Biology
Großzebrafisch Embryonale Herz Dissection für Transkriptionsanalyse
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Großzebrafisch Embryonale Herz Dissection für Transkriptionsanalyse

Article DOI: 10.3791/52087-v • 10:00 min • January 12th, 2015
January 12th, 2015 •
*1,2,3, *1,2, 1,2,3
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology, University of Potsdam, 3Institute of Molecular Biology, Medizinische Hochschule Hannover
* These authors contributed equally

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Herzgenexpressionsprofile bei Zebrafisch Herzentwicklung zu analysieren, wurde Gesamt-RNA aus isolierten Herzen gewonnen werden. Hier präsentieren wir ein Protokoll für das Sammeln von Funktions / klopfenden Herzen durch schnelle manuelle Dissektion von Zebrafischembryonen zu herzspezifischen mRNA zu erhalten.

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Entwicklungsbiologie Zebrafisch Embryo herz Dissektion RNA RT-qPCR
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