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Longo prazo Time Lapse Imagem do mouse cocleares explantes
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Longo prazo Time Lapse Imagem do mouse cocleares explantes

Article DOI: 10.3791/52101-v • 10:43 min • November 2nd, 2014
November 2nd, 2014 •
1, 1,2,3
1Biological Sciences Platform, Sunnybrook Research Institute, 2Department of Otolaryngology - Head and Neck Surgery, University of Toronto, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

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Imagens ao vivo da cóclea de mamíferos embrionária é um desafio porque os processos de desenvolvimento na mão operam em um gradiente temporal, ao longo de dez dias. Aqui é apresentado um método para a cultura e, em seguida, imagiologia embrionário tecido de explante da cóclea retirado de um rato repórter fluorescente ao longo de cinco dias.

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Bioengenharia o Live-imaging lapso de tempo cóclea orelha repórter do mouse desenvolvimento incubadora microscópio Sox2
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